CUET UG Categorised PYQ Biology Unit 8
BIOLOGY
📌 Answers are locked once submitted — results and explanations appear at the end.
QUESTION 1 OF 19
Match List – I with List – II (PYQ 2022 Shift 1)
| List – I | List – II |
|---|---|
| (A) Streptokinase | (I) Blood-Cholesterol lowering agents |
| (B) Cyclosporin A | (II) Clot Buster |
| (C) Statins | (III) Propionibacterium sharmanii |
| (D) Swiss Cheese | (IV) Immuno suppressive agent |
QUESTION 2 OF 19
Given below are two statements: (PYQ 2022 Shift 1)
Statement I: Ladybird and Dragonflies are useful to get rid of aphids and mosquitoes. Trichoderma fungus are effective bio control agents of several plant pathogens.
Statement II: The biological control of plant diseases and pest can control increasing use of insecticides and pesticides, thus saving our environment from being polluted.
QUESTION 3 OF 19
Propionibacterium sharmanii is used in making which of the following food item? (PYQ 2023 Shift 1)
QUESTION 4 OF 19
Which of the following is used as 'clot buster'? (PYQ 2023 Shift 1)
QUESTION 5 OF 19
Match the following: (PYQ 2023 Shift 1)
| List I | List II |
|---|---|
| A. Aspergillus niger | I. Butyric acid |
| B. Clostridium butylicum | II. Acetic acid |
| C. Saccharomyces cerevisiae | III. Citric acid |
| D. Acetobacter aceti | IV. Ethanol |
QUESTION 6 OF 19
Match the following: (PYQ 2023 Shift 2)
| List I | List II |
|---|---|
| A. Aspergillus niger | I. Cyclosporin-A |
| B. Trichoderma polysporum | II. Ethanol |
| C. Lactobacillus | III. Citric acid |
| D. Saccharomyces cerevisiae | IV. Lactic acid |
QUESTION 7 OF 19
Which of the following acts as biofertilizer in paddy field? (PYQ 2023 Shift 2)
QUESTION 8 OF 19
The puffed-up appearance of dough is due to the production of : (PYQ 2023 Shift 2)
QUESTION 9 OF 19
Which of the following microbe is used for making bread? (PYQ 2023 Shift 3)
QUESTION 10 OF 19
Baculoviruses attack on: (PYQ 2023 Shift 3)
QUESTION 11 OF 19
Match List I with List II (PYQ 2023 Shift 3)
| List I | List II |
|---|---|
| A. Methanobacterium | I. Butyric acid |
| B. Monascus purpureus | II. Breakdown of cellulose |
| C. Clostridium butylicum | III. Clot buster |
| D. Streptococcus | IV. Blood-cholesterol lowering agent |
QUESTION 12 OF 19
Which of the following is not used as Single Cell Protein (SCP)? (PYQ 2023 Shift 3)
QUESTION 13 OF 19
Streptokinase enzyme is used for: (PYQ 2024 Shift 1, 2024 Shift 2)
QUESTION 14 OF 19
Which of the following plays a significant role in our stomach in checking disease causing microbes? (PYQ 2024 Shift 1, 2024 Shift 2)
QUESTION 15 OF 19
Given below are two statements: (PYQ 2024 Shift 1, 2024 Shift 2)
Statement I: Whisky, brandy and rum are produced without distillation of the fermented broth.
Statement II: Saccharomyces cerevisiae is called doctor's yeast.
QUESTION 16 OF 19
Match the following: (PYQ 2025 Shift 1)
| List I | List II |
|---|---|
| A. Clostridium butylicum | I. Lactic acid |
| B. Aspergillus niger | II. Butyric acid |
| C. Acetobacter aceti | III. Citric acid |
| D. Lactobacillus | IV. Acetic acid |
QUESTION 17 OF 19
Match the following: (PYQ 2025 Shift 1)
| Column A (Product) | Column B (Producer) |
|---|---|
| A. Citric Acid | I. Trichoderma polysporum |
| B. Ethanol | II. Monascus purpureus |
| C. Statins | III. Saccharomyces cerevisiae |
| D. Cyclosporin A | IV. Aspergillus niger |
QUESTION 18 OF 19
Baculoviruses are pathogens that attack: (PYQ 2025 Shift 1)
QUESTION 19 OF 19
Arrange the given products formed during sewage treatment in correct sequence: (PYQ 2025 Shift 1)
(A) Biogas
(B) Activated sludge
(C) Flocs
(D) Primary sludge
Test Complete!
Answer Review
1 Match List – I with List – II (PYQ 2022 Shift 1)
| List – I | List – II |
|---|---|
| (A) Streptokinase | (I) Blood-Cholesterol lowering agents |
| (B) Cyclosporin A | (II) Clot Buster |
| (C) Statins | (III) Propionibacterium sharmanii |
| (D) Swiss Cheese | (IV) Immuno suppressive agent |
Streptokinase → Clot buster. Cyclosporin A → Immunosuppressant. Statins → Lower cholesterol. Swiss cheese → Propionibacterium.
The correct matching is: • Streptokinase → Clot buster • Cyclosporin A → Immunosuppressive agent • Statins → Blood cholesterol lowering agents • Swiss cheese → Propionibacterium sharmanii
- Option A) Statins incorrectly matched.
- Option C) Multiple mismatches.
- Option D) Multiple mismatches.
Used: Option Grouping
- Option A → Incorrect.
- Option B → Correct.
- Option C → Incorrect.
- Option D → Incorrect.
- Final Answer → Option B.
2 Given below are two statements: (PYQ 2022 Shift 1)
Statement I: Ladybird and Dragonflies are useful to get rid of aphids and mosquitoes. Trichoderma fungus are effective bio control agents of several plant pathogens.
Statement II: The biological control of plant diseases and pest can control increasing use of insecticides and pesticides, thus saving our environment from being polluted.
Ladybirds control aphids. Dragonflies control mosquitoes. Trichoderma is a biocontrol agent.
Statement I is correct because ladybird beetles prey on aphids, dragonflies help control mosquitoes and Trichoderma species act as biological control agents against plant pathogens. Statement II is also correct because biological control reduces dependence on chemical pesticides, thereby reducing environmental pollution.
- Option B) Both statements are actually correct.
- Option C) Statement II is also correct.
- Option D) Statement I is correct.
Used: NCERT Recall
- Option A → Correct.
- Option B → Incorrect.
- Option C → Statement II correct.
- Option D → Statement I correct.
- Final Answer → Option A.
3 Propionibacterium sharmanii is used in making which of the following food item? (PYQ 2023 Shift 1)
Produces CO₂. Creates characteristic holes. Used in Swiss cheese production.
Propionibacterium sharmanii is used during Swiss cheese manufacture. It releases carbon dioxide, forming the characteristic large holes and imparting a distinct flavour.
- Option B) Roquefort cheese uses Penicillium roqueforti.
- Option C) Bread uses Saccharomyces cerevisiae.
- Option D) Toddy is produced by natural fermentation of palm sap.
Used: NCERT Recall
- Option A → Correct organism-product pair.
- Option B → Different microbe.
- Option C → Yeast-based product.
- Option D → Fermented beverage.
- Final Answer → Swiss cheese.
4 Which of the following is used as 'clot buster'? (PYQ 2023 Shift 1)
Dissolves blood clots. Produced by Streptococcus. Used during myocardial infarction.
Streptokinase is a thrombolytic enzyme used to dissolve blood clots in blood vessels. It is often administered in cases of heart attack and other thrombotic disorders.
- Option B) Immunosuppressant drug.
- Option C) Cholesterol-lowering drug.
- Option D) Antibiotic.
Used: Elimination
- Option A → Clot dissolver.
- Option B → Organ transplant drug.
- Option C → Lipid lowering.
- Option D → Antibacterial drug.
- Final Answer → Streptokinase.
5 Match the following: (PYQ 2023 Shift 1)
| List I | List II |
|---|---|
| A. Aspergillus niger | I. Butyric acid |
| B. Clostridium butylicum | II. Acetic acid |
| C. Saccharomyces cerevisiae | III. Citric acid |
| D. Acetobacter aceti | IV. Ethanol |
Aspergillus niger → Citric acid. Clostridium butylicum → Butyric acid. Yeast → Ethanol. Acetobacter → Acetic acid.
Industrial microbiology utilizes Aspergillus niger for citric acid production, Clostridium butylicum for butyric acid, Saccharomyces cerevisiae for ethanol and Acetobacter aceti for acetic acid production.
- Option A) Product-organism mismatches.
- Option C) Incorrect assignments.
- Option D) Multiple wrong matches.
Used: Option Grouping
- Option A → Incorrect.
- Option B → All correct matches.
- Option C → Incorrect.
- Option D → Incorrect.
- Final Answer → Option B.
6 Match the following: (PYQ 2023 Shift 2)
| List I | List II |
|---|---|
| A. Aspergillus niger | I. Cyclosporin-A |
| B. Trichoderma polysporum | II. Ethanol |
| C. Lactobacillus | III. Citric acid |
| D. Saccharomyces cerevisiae | IV. Lactic acid |
Aspergillus niger → Citric acid. Trichoderma polysporum → Cyclosporin-A. Lactobacillus → Lactic acid. Yeast → Ethanol.
Aspergillus niger produces citric acid, Trichoderma polysporum produces Cyclosporin-A, Lactobacillus produces lactic acid and Saccharomyces cerevisiae produces ethanol.
- Option B) Product assignments incorrect.
- Option C) Lactobacillus mismatched.
- Option D) Multiple incorrect associations.
Used: Option Grouping
- Option A → Correct.
- Option B → Incorrect.
- Option C → Incorrect.
- Option D → Incorrect.
- Final Answer → Option A.
7 Which of the following acts as biofertilizer in paddy field? (PYQ 2023 Shift 2)
Cyanobacteria used in paddy fields. Fixes atmospheric nitrogen. Improves soil fertility.
Nostoc is a nitrogen-fixing cyanobacterium widely used as a biofertilizer in paddy fields. It enriches soil nitrogen and improves crop productivity.
- Option B) Associated mainly with legumes.
- Option C) Used in cereals but not the NCERT paddy-field example.
- Option D) Free-living nitrogen fixer.
Used: NCERT Recall
- Option A → Correct.
- Option B → Legumes.
- Option C → Non-specific.
- Option D → Different application.
- Final Answer → Nostoc.
8 The puffed-up appearance of dough is due to the production of : (PYQ 2023 Shift 2)
Yeast ferments sugars. CO₂ gets trapped in dough. Dough expands.
During fermentation, yeast converts sugars into alcohol and carbon dioxide. The trapped carbon dioxide gas causes the dough to rise and become puffed up.
- Option B) Produced, but does not cause puffing.
- Option C) Not responsible.
- Option D) Not produced by fermentation.
Used: NCERT Recall
- Option A → Correct.
- Option B → Secondary product.
- Option C → Incorrect.
- Option D → Incorrect.
- Final Answer → Carbon dioxide.
9 Which of the following microbe is used for making bread? (PYQ 2023 Shift 3)
Commonly called baker's yeast. Produces carbon dioxide. Causes dough to rise.
Saccharomyces cerevisiae (baker's yeast) ferments sugars present in dough and releases carbon dioxide. The trapped gas makes the dough rise and gives bread its soft texture.
- Option A) Used in Swiss cheese production.
- Option C) Produces citric acid.
- Option D) Produces Cyclosporin-A.
Used: NCERT Recall
- Option A → Cheese industry.
- Option B → Bread making.
- Option C → Citric acid.
- Option D → Immunosuppressant production.
- Final Answer → Saccharomyces cerevisiae.
10 Baculoviruses attack on: (PYQ 2023 Shift 3)
Used as biological control agents. Infect insects and arthropods. Safe for vertebrates.
Baculoviruses are pathogens that specifically infect arthropods, especially insects. They are widely used in biological pest control because they do not harm plants, mammals, birds or fishes.
- Option A) Birds are not hosts.
- Option C) Mammals are not affected.
- Option D) Fishes are not hosts.
Used: NCERT Recall
- Option A → Incorrect.
- Option B → Correct.
- Option C → Incorrect.
- Option D → Incorrect.
- Final Answer → Arthropods.
11 Match List I with List II (PYQ 2023 Shift 3)
| List I | List II |
|---|---|
| A. Methanobacterium | I. Butyric acid |
| B. Monascus purpureus | II. Breakdown of cellulose |
| C. Clostridium butylicum | III. Clot buster |
| D. Streptococcus | IV. Blood-cholesterol lowering agent |
Methanobacterium → Cellulose breakdown. Monascus → Statins. Clostridium → Butyric acid. Streptococcus → Clot buster.
Methanobacterium helps in cellulose digestion. Monascus purpureus produces statins that lower blood cholesterol. Clostridium butylicum produces butyric acid. Streptococcus produces streptokinase, a clot buster.
- Option A) Multiple mismatches.
- Option B) Monascus incorrectly matched.
- Option D) Incorrect assignments.
Used: Option Grouping
- Option A → Incorrect.
- Option B → Incorrect.
- Option C → Correct.
- Option D → Incorrect.
- Final Answer → Option C.
12 Which of the following is not used as Single Cell Protein (SCP)? (PYQ 2023 Shift 3)
SCP includes microbes and microalgae. Chlorella and Spirulina are SCP sources. Gracilaria is mainly used for agar production.
Methylophilus methylotrophus, Chlorella and Spirulina are well-known sources of Single Cell Protein. Gracilaria is a red alga primarily used for agar extraction and is not commonly used as SCP.
- Option A) SCP source.
- Option B) SCP source.
- Option C) SCP source.
Used: Odd One Out
- Option A → SCP.
- Option B → SCP.
- Option C → SCP.
- Option D → Agar-producing alga.
- Final Answer → Gracilaria.
13 Streptokinase enzyme is used for: (PYQ 2024 Shift 1, 2024 Shift 2)
Clot-buster enzyme. Produced by Streptococcus. Used in heart attack treatment.
Streptokinase acts as a thrombolytic agent. It dissolves blood clots by activating plasminogen into plasmin and is used in patients suffering from myocardial infarction.
- Option B) Pectinase is used for fruit juice clarification.
- Option C) Not related to cholesterol synthesis.
- Option D) Lipases are used for oily stain removal.
Used: Elimination
- Option A → Medical clot removal.
- Option B → Different enzyme.
- Option C → Incorrect function.
- Option D → Different enzyme.
- Final Answer → Option A.
14 Which of the following plays a significant role in our stomach in checking disease causing microbes? (PYQ 2024 Shift 1, 2024 Shift 2)
Beneficial bacterium. Produces acids. Suppresses harmful microbes.
Lactobacillus is a probiotic bacterium that helps maintain microbial balance in the digestive tract and inhibits disease-causing microorganisms.
- Option A) Produces penicillin.
- Option B) Produces statins.
- Option C) Produces cyclosporin A.
Used: Odd One Out
- Option A → Antibiotic producer.
- Option B → Statin producer.
- Option C → Immunosuppressant producer.
- Option D → Gut microflora.
- Final Answer → Lactobacillus.
15 Given below are two statements: (PYQ 2024 Shift 1, 2024 Shift 2)
Statement I: Whisky, brandy and rum are produced without distillation of the fermented broth.
Statement II: Saccharomyces cerevisiae is called doctor's yeast.
Alcoholic beverages require distillation. Saccharomyces cerevisiae is brewer's yeast. Both statements are incorrect.
Whisky, rum and brandy are produced by distillation of fermented broth. Saccharomyces cerevisiae is known as brewer's yeast, not doctor's yeast. Therefore both statements are false.
- Option A) Both statements are not true.
- Option C) Statement I is false.
- Option D) Statement II is false.
Used: Elimination
- Option A → Incorrect.
- Option B → Both false.
- Option C → First statement false.
- Option D → Second statement false.
- Final Answer → Option B.
16 Match the following: (PYQ 2025 Shift 1)
| List I | List II |
|---|---|
| A. Clostridium butylicum | I. Lactic acid |
| B. Aspergillus niger | II. Butyric acid |
| C. Acetobacter aceti | III. Citric acid |
| D. Lactobacillus | IV. Acetic acid |
Clostridium → Butyric acid. Aspergillus → Citric acid. Acetobacter → Acetic acid. Lactobacillus → Lactic acid.
Industrial microbiology uses specific microbes for commercial products. Clostridium butylicum produces butyric acid, Aspergillus niger produces citric acid, Acetobacter aceti produces acetic acid, and Lactobacillus produces lactic acid during fermentation. These are standard NCERT examples.
- Option A) Most producer-product combinations are incorrect.
- Option C) Clostridium and Aspergillus are mismatched.
- Option D) Nearly all pairings are wrong.
Used: NCERT Recall
- Option A → Incorrect pairing.
- Option B → Complete correct matching.
- Option C → Wrong acids assigned.
- Final Answer → Based on NCERT recall.
17 Match the following: (PYQ 2025 Shift 1)
| Column A (Product) | Column B (Producer) |
|---|---|
| A. Citric Acid | I. Trichoderma polysporum |
| B. Ethanol | II. Monascus purpureus |
| C. Statins | III. Saccharomyces cerevisiae |
| D. Cyclosporin A | IV. Aspergillus niger |
Aspergillus → Citric acid. Yeast → Ethanol. Monascus → Statins. Trichoderma → Cyclosporin A.
Aspergillus niger is used commercially for citric acid production. Saccharomyces cerevisiae (yeast) produces ethanol through fermentation. Monascus purpureus produces statins, which help lower blood cholesterol levels. Trichoderma polysporum produces cyclosporin A, an immunosuppressive agent used during organ transplantation.
- Option B) Citric acid and cyclosporin A producers are incorrectly matched.
- Option C) Several microbial products are wrongly assigned.
- Option D) Most producer-product combinations are incorrect.
Used: NCERT Recall
- Option A → Exact NCERT matching.
- Option B → Citric acid mismatch.
- Option C → Multiple mismatches.
- Final Answer → Based on NCERT recall.
18 Baculoviruses are pathogens that attack: (PYQ 2025 Shift 1)
Baculoviruses infect insects. Used as biological control agents. Environment-friendly pest control.
Baculoviruses are insect-specific pathogens widely used in biological pest control programs. The most commonly used species belong to the genus Nucleopolyhedrovirus (NPV). They are highly specific to insects and do not harm beneficial organisms, humans, or other vertebrates.
- Option B) Baculoviruses do not infect nematodes.
- Option C) Not the target hosts of baculoviruses.
- Option D) Birds are not infected by baculoviruses.
Used: NCERT Recall
- Option A → Correct host group.
- Option B → Incorrect organism group.
- Option C → Not targeted.
- Final Answer → Based on NCERT recall.
19 Arrange the given products formed during sewage treatment in correct sequence: (PYQ 2025 Shift 1)
(A) Biogas
(B) Activated sludge
(C) Flocs
(D) Primary sludge
Primary treatment forms primary sludge. Secondary treatment forms flocs. Activated sludge is produced. Anaerobic digestion generates biogas.
During primary treatment, suspended solids settle and form primary sludge. In secondary treatment, microbial masses called flocs develop and degrade organic matter. Part of the flocs settle to form activated sludge. Activated sludge is then transferred to anaerobic digesters where methanogenic bacteria produce biogas. Thus, the correct sequence is D → C → B → A.
- Option A) Reverses the actual treatment sequence.
- Option C) Flocs cannot form before primary sludge.
- Option D) Places activated sludge and biogas before treatment begins.
Used: Sequential Biology Process
- Option A → Reverse order.
- Option B → Matches sewage treatment sequence.
- Option C → Incorrect starting step.
- Final Answer → Based on process order.
